Biomedical Reseach Lab

The Biomedical Research Lab covers almost 900 square meters and is structured in both general open spaces, plus isolated rooms for more specialized operations.

Our researchers aim to understand the molecular events occurring in disease, identifying and modelling specific genetic mutations. To do this they first rely on statistical and bioinformatic approaches, including genome-wide linkage and association studies and next-generation sequencing techniques, and multi-omic profiling to identify genes/mutations and pathways of interest. Once genetic changes that contribute to the development of disease have been identified, researchers then use cellular models to try to understand what is taking place in the cells as a result of those genetic variations.

Many technologies in the laboratory support this work, from simple DNA and protein analysis up to more complicated molecular characterizations. Central to our research are advanced Model systems that replicate human tissues and disease mechanisms. In our work with human cell models, we move beyond traditional 2D cultures to create advanced 3D systems that replicate the complexity of human tissues. These include organoids, which are miniature organ-like structures grown from stem cells; assembloids, which combine multiple organoids or cell types to simulate interactions between organs; engineered human tissues that reproduce the architecture of real tissues; and micro-physiological systems—often called organ-on-a-chip—microfluidic devices that mimic key physiological functions. These models can be derived from reference cell lines, biobanks of patient cells, cells genetically edited using CRISPR/Cas9 technology, or sourced from our own biobank of over 13,000 individuals that have participated in our ongoing population health study.

Electrophysiology represents another cornerstone of our work. Using MEA (Microelectrode Array) systems, and standard single-cell (patch- and voltage-clamp), we study the electrical properties of the cellular models we generate. This approach allows us to investigate neuronal activity, cardiac function, and other electrically active systems, providing crucial insights into disease mechanisms and potential therapeutic strategies.

Finally, microscopy enables us to visualize cellular and tissue-level changes with exceptional precision. Our imaging suite currently includes a Leica SP8 confocal microscope with white-light laser for high-resolution static and live-cell imaging, and a Nikon Ti2‐E Inverted Microscope, equipped for both widefield and confocal capabilities. The widefield function of the Nikon enables large field of view acquisition with a DS‐Qi2 camera, while the confocal function utilizes a spinning disk system, LED lines illumination and a Lumencor Spectra X Chroma camera. We have recently added a new Opera Phenix Plus high-content imaging system, which integrates advanced spectral imaging and ultra-fast scanning capabilities for complex biological samples.