ANCIENT PATHOGENS

Molecular analysis of the microbiome and of infectious diseases in ancient human remains - from capture-sequencing to metagenomics diagnostics.

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  • Project duration: December 2012 - December 2022
  • Project status:
    Approval by the Scientific Committee
  • Funding:
    Internal funding EURAC (Project)
  • Total project budget: €530,000.00

Ancient skeletal and soft tissue samples can still contain traces of the original bacterial DNA. This applies both to the DNA of human commensals (e.g. intestinal bacteria) and to the genetic material of pathogens. Thereby, genome wide analysis of ancient pathogens provides information on the onset and spread of chronic infectious diseases such as tuberculosis or leprosy and helps to identify the causative agents of major human epidemics such as the plague. Furthermore, the metagenomic study of ancient human microbiomes provide novel insights into the composition and possible changes of human microbial communities over the last millennia. The biomolecular investigation of pathogens in ancient human remains represents an emerging field of research which provides considerable insight into the history and evolution of infectious diseases. The applied diagnostic approaches in paleomicobiology range from the histological detection to the PCR-based characterization of ancient pathogens. More recently, the era of high-throughput sequencing paved new ways to analyze ancient DNA resulting e.g. in the first complete genome of the causative agent of Black Death, Yersinia pestis. However there are obstacles yet to overcome such as the limitations of PCR-based approaches in analyzing highly degraded ancient DNA extracts or the development of bioinformatics pipelines to screen complex metagenomic datasets for ancient pathogens.

The aim of this project will be to improve the DNA-based detection of the microbiome incl. pathogens in ancient human remains. To further optimize molecular diagnostics approaches the EURAC Institute for Mummy Studies has access to an variety of tissue samples with possible tuberculosis, brucellosis and malaria infection. Moreover, together with our cooperation partner from Vienna and Stockholm we are analyzing the metagenomic data of the Iceman´s genomic survey in order to establish a bioinformatics pipeline for the de novo detection of commensals and pathogens in metagenomic datasets of ancient human remains. Thereby obtained knowledge is currently also applied to shotgun datasets of Tuberculosis positive skeletal material. In addition we seek for molecular traces of other diseases such as e.g. leprosy, syphilis, malaria and plague in ancient human remains.

Publications
Verification of tuberculosis infection among Vác mummies (18th century CE, Hungary) based on lipid biomarker profiling with a new HPLC-HESI-MS approach
Váradi OA, Rakk D, Spekker O, Terhes G, Urbán E, Berthon W, Pap I, Szikossy I, Maixner F, Zink A, Vágvólgyi C, Donoghue H, Minnikin D, Szekeres A, Pálfi G (2021)
Journal article
Tuberculosis

More information: https://doi.org/10.1016/j.tube.2020.102037

https://doi.org/10.1016/j.tube.2020.102037

http://hdl.handle.net/10863/16368

Ancient DNA analysis of rare genetic bone disorders
Maixner F, Gresky J, Zink A (2021)
Journal article
International Journal of Paleopathology

More information: https://doi.org/10.1016/j.ijpp.2021.04.009

https://doi.org/10.1016/j.ijpp.2021.04.009

http://hdl.handle.net/10863/17193

Reconstruction of ancient microbial genomes from the human gut
Wibowo MC, Yang Z, Borry M, Hübner A, Huang KD, Tierney BT, Zimmerman S, Barajas-Olmos F, Contreras-Cubas C, García-Ortiz H, Martínez-Hernández A, Luber JM, Kirstahler P, Blohm T, Smiley FE, Arnold R, Ballal SA, Pamp SJ, Russ J, Maixner F, Rota-Stabelli O, Segata N, Reinhard K, Orozco L, Warinner C, Snow M, LeBlanc S, Kostic AD (2021)
Journal article
Nature

More information: https://www.nature.com/articles/s41586-021-03532-0

https://doi.org/10.1038/s41586-021-03532-0

http://hdl.handle.net/10863/17191

Recovery of ancient human- and microbial DNA from cave bones and-sediments
Sarhan M, Lehmkuhl A, Wieland G, Franken M, Zink A, Maixner F (2021)
Presentation/Speech

Conference: Bioinformatics Virtual Coordination Network Virtual Conference| Holistic Bioinformatic Approaches used in Microbiome Research | : 7.6.2021 - 11.6.2021

Molecular and microscopic analysis of human paleofeces from the salt mines in Hallstatt, Austria, provide precious insights into dietary habits and gut microbiome composition of Iron Age miners
Maixner F, Sarhan MS, Huang KD, Tett A, Schoenafinger A, Zingale S, Blanco-Míguez A, Manghi P, Cemper-Kiesslich J, Rosendahl W, Kusebauch U, Morrone SR, Hoopmann MR, Rota-Stabelli O, Rattei T, Moritz RL, Oeggl K, Segata N, Zink A, Reschreiter H, Kowarik K (2021)
Presentation/Speech

Conference: ISBA9| 9TH INTERNATIONAL SYMPOSIUM ON BIOMOLECULAR ARCHAEOLOGY | Toulouse : 1.6.2021 - 4.6.2021

Lipid biomarker based verification of TB infection in mother’s and daughter’s mummified human remains (Vác Mummy Collection, 18th century, CE, Hungary)
Váradia OA, Szikossy I, Spekkera O, Rakk D, Terhes G, Urbán E, Berthon W, Pap I, Maixner F, Zink A, Vágvölgyi C, Donoghue HD, Minnikin DE, Pálfi´G, Szekeres A (2020)
Journal article
Acta Biologica Szegediensis

More information: http://abs.bibl.u-szeged.hu/index.php/abs

https://doi.org/10.14232/abs.2020.2.99-109

Analysis of 1,321 Eubacterium rectale genomes from metagenomes uncovers complex phylogeographic population structures and subspecies functional adaptations
Karcher N, Pasolli E, Asnicar F, Huang K, Tett A, Manara S, Armanini F, Bain D, Duncan SH, Louis P, Zolfo M, Manghi P, Raffaetà R, Rota-Stabelli O, Collado MC, Zeller G, Falush D, Maixner F, Walker AW, Huttenhower C, Segata N (2020)
Journal article
Genome Biology

https://doi.org/10.1186/s13059-020-02042-y

http://hdl.handle.net/10863/14778

Helicobacter pylori in ancient human remains
Maixner F, Thorell K, Granehäll L, Linz B, Moodley Y, Rattei T, Engstrand L, Zink A (2019)
Journal article
World Journal of Gastroenterology

More information: https://www.wjgnet.com/1007-9327/full/v25/i42/6289.htm

https://doi.org/10.3748/wjg.v25.i42.6289

http://hdl.handle.net/10863/12256

Molecular Reconstruction of the Diet in Human Stool Samples
Maixner F (2019)
Journal article
mSystems

https://doi.org/10.1128/mSystems.00634-19

http://hdl.handle.net/10863/12257

The Prevotella copri complex comprises four distinct clades that are underrepresented in Westernized populations
Tett A, Huang KD, Asnicar F, Fehlner-Peach H, Pasolli E, Karcher N, Armanini F, Manghi P, Bonham K, Zolfo M, De Filippis F, Magnabosco C, Bonneau R, Lusingu R, Amuasi J, Reinhard K, Rattei T, Boulund F, Engstrand L, Zink A, Collado MC, Littman DR, Eibach D, Ercolini D, Rota-Stabelli O, Huttenhower C, Maixner F, Segata N (2019)
Journal article
Cell Host and Microbe

https://doi.org/10.1016/j.chom.2019.08.018

http://hdl.handle.net/10863/11462

The comparative study of two extraction methods for ancient DNA: silica suspension method and ultracentrifugal concentrator method
Lee EJ, Maixner F, Zink A (2018)
Journal article
Analytical Science & Technology

https://doi.org/10.5806/AST.2018.31.2.65

http://hdl.handle.net/10863/6824

The Iceman's Last Meal Consisted of Fat, Wild Meat, and Cereals
Maixner F, Turaev D, Cazenave-Gassiot A, Janko M, Krause-Kyora B, Hoopmann MR, Kusebauch U, Sartain M, Guerriero G, O'Sullivan N, Teasdale M, Cipollini G, Paladin A, Mattiangeli V, Samadelli M, Putzer A, Tecchiati U, Meissen J, Palazoglu M, Rausch P, Lösch S, Kim BJ, Baines JF, Gostner P, An HJ, Malfertheiner P, Egarter-Vigl E, Stark RW, Keller A, Bishop D, Wenk M, Engstrand L, Moritz RL, Bradley DG, Fiehn O, Nebel A, Oeggl K, Doble P, Franke A, Rattei T, Grimm R, Zink A (2018)
Journal article
Current Biology

https://doi.org/10.1016/j.cub.2018.05.067

http://hdl.handle.net/10863/6829

miRNAs in ancient tissue specimens of the Tyrolean Iceman
Keller A, Kreis S, Leidinger P, Maixner F, Ludwig N, Backes C, Galata V, Guerriero G, Fehlmann T, Franke A, Meder B, Zink A, Meese E (2017)
Journal article
Molecular Biology and Evolution

https://doi.org/10.1093/molbev/msw291

http://hdl.handle.net/10863/7334

Tuberculosis in early medieval Switzerland: osteological and molecular evidence from Courroux
Cooper C, Fellner R, Heubi O, Maixner F, Zink A, Lösch S (2016)
Journal article
Swiss Medical Weekly

https://doi.org/10.4414/smw.2016.14269

http://hdl.handle.net/10863/6670

The 5300-year-old Helicobacter pylori genome of the Iceman
Maixner F, Krause-Kyora B, Turaev D, Herbig A, Hoopmann MR, Hallows JL, Kusebauch U, Vigl EE, Malfertheiner P, Megraud F, O'Sullivan N, Cipollini G, Coia V, Samadelli M, Engstrand L, Linz B, Moritz RL, Grimm R, Krause J, Nebel A, Moodley Y, Rattei T, Zink A (2016)
Journal article
Science (New York, N.Y.)

https://doi.org/10.1126/science.aad2545

http://hdl.handle.net/10863/6124

Partner
University College Dublin, School of Archeology
University of Tübingen
Reiss-Engelhorn-Museen Mannheim
Max Planck Institute for the Science of Human History
Department of Biological Anthropology, University of Szeged, Dugonics Ter, Szeged, Hungary
University of Bern, Department of Physical Anthropology, Institute of Forensic Medicine
Medizinische Universität Wien, Forensische Medizin, Fachbereich Forensische Anthropologie
Centre for Translational Microbiome Research (CTMR), Karolinska Institute, Stockholm, Sweden
University of Vienna, CUBE - Division of Computational Systems Biology, Department of Microbiology and Ecosystem Science
Universita degli Studi di Firenze, Facultà di Medicina e Chirurgia
University of Trento, Centre for Integrative Biology
Project Team
1 - 2

Projects

1 - 7
Project

MummyLabs

Development and centralization of the laboratory infrastructures of the Institute for Mummy Studies ...

Duration: December 2017 - October 2021Funding:
FESR (EU funding / Project)

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Institute's Projects

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Eurac Research is a private research center based in Bolzano (South Tyrol) with researchers from a wide variety of scientific fields who come from all over the globe. Together, through scientific knowledge and research, they share the goal of shaping the future.

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