PopGenCT Ancient Egypt

In questo progetto, studieremo la storia genetica della popolazione egizia utilizzando analisi del DNA antico (aDNA). A tal fine, verranno analizzate mummie e resti scheletrici egiziani conservati nelle collezioni del Museo Egizio di Torino e del Museo Egizio e della Collezione di Papiri del museo nazionale di Berlino (“Staatliche Museen zu Berlin”). I resti studiati coprono un arco temporale di circa 5000 anni (4000 a.C. - 800 d.C.), e sono stati recuperati da diversi siti archeologici in Egitto. Pertanto, i dati ottenuti da questo progetto copriranno, per la prima volta, tutti i periodi dell'antica storia egizia e inoltre un ampio contesto geografico. Tutti gli individui saranno sottoposti a metodi di sequenziamento di nuova generazione. Inoltre, la tecnica di arricchimento del DNA permetterà di ricostruire il genoma mitocondriale (mtDNA) degli individui sotto esame, e, per coloro presentanti una buona conservazione del DNA, si cercherà di approfondire le analisi dell´ intero genoma. In questo modo, studieremo la storia genetica dell’Egitto non solo a livello uniparentale (mtDNA), ma anche su scala del genoma nucleare.

Inoltre, in collaborazione con l'Università di Losanna, Svizzera, alcune mummie saranno esaminate tramite tomografia computerizzata (TC), con lo scopo di effettuare un test metodologico. In particolare, il test mira a valutare l’applicabilità di due metodi di determinazione del sesso alle scansioni TC, l'Indice Ischio-Pubico (IPI) e la “Diagnose Sexuelle Probabiliste” (DSP). Questo confronto fornirà ulteriori approfondimenti sulle possibilità e sui limiti degli esami basati sulla TC dei resti umani mummificati.

Ancient DNA (aDNA) research

Egypt has been home to one of the earliest great human civilizations. Throughout its history spanning several millennia, this civilization had numerous interactions with populations in Africa, Europe, and western Asia, including international trade, military campaigns, foreign invasions, and foreign rule. Apart from cultural influences, these interactions likely also shaped the genetic composition of Egypt’s population and thus make Egypt an interesting setting for population genetic studies.

However, only during the last decade, due to technological improvements, such as the development of high-throughput sequencing (HTS) methods, genetic studies have succeeded in the retrieval and assessment of authentic aDNA obtained from ancient Egyptian mummies and skeletal material. As a result, genetic data for the contemporary population of ancient Egypt remains scarce. In addition, available genome datasets date almost exclusively to periods after the New Kingdom (1550 - 1064 BC), resulting in a lack of information for the older periods of ancient Egyptian history. Furthermore, these datasets have been obtained almost exclusively from a single archaeological site in northern Egypt and may thus not be representative for all of ancient Egypt.

To address these issues, this project will focus on a large-scale genetic study on the collections of the Museo Egizio of Turin and the Egyptian Museum and Papyrus Collection of the Staatliche Museen zu Berlin, comprising approximately 150 to 200 ancient Egyptian mummies and skeletal remains. A selection of these specimens, encompassing a time span of approximately 5000 years (4000 BC - AD 800) as well as different archaeological finding sites, will be analyzed using HTS methods, including the application of hybridization capture techniques for targeted DNA enrichment. Initial analyses will focus on the enrichment and assessment of the mitochondrial DNA (mtDNA) for the reconstruction of mitochondrial genomes. Afterwards, nuclear DNA enrichments will be applied to specimens exhibiting a good DNA preservation to also reconstruct whole-genome datasets.

Thus, this project will investigate Egypt's population genetic history not only uniparentally, based on the maternal lineage, but also on a genome-wide level. Furthermore, the genomes obtained by this project will expand the pool of currently available datasets and will provide novel information for the older periods of Egypt’s past as well as for a broader geographical context.

Anthropological analysis using CT scans

Computed tomography (CT) constitutes an examination method, which permits the assessment of archaeological remains, for which the bones are not readily accessible (e.g., fully wrapped mummies).

Using methods from physical anthropology it is thus possible to reconstruct an individual’s biological (age at death, sex, stature) and paleopathological profile, without inflicting damage to the investigated specimen. However, it has been shown that the accuracy of anthropological age at death estimation methods, which were developed for dry bones, can decrease upon their application to virtual bone models created from CT scans.

Therefore, the second part of this project aims to investigate the occurrence of similar issues in methods used for sex determination. For this, some of the mummies from the museums’ collections will be examined by means of computed tomography in the framework of a methodological test. This test will be performed in collaboration with our partners at the University of Lausanne, Switzerland, and will assess the applicability of two sexing methods, the Ischio-Pubic Index (IPI) and the Diagnose Sexuelle Probabiliste (DSP), to CT scans of ancient Egyptian mummies.

Thereby, this study will provide further insights into the possibilities and limitations of CT-based studies on mummified human remains.

Related research projects

The population genetic study as well as the methodological test performed within this project constitute an addition to the interdisciplinary scientific investigation of the collections of the Museo Egizio of Turin and the Egyptian Museum and Papyrus Collection of the Staatliche Museen zu Berlin in the framework of the “Mummy Atherosclerosis” project and the “ME Torino Mummy Project”. Thus, the present study can not only benefit from the material (sample material for the genetic analysis, CT scans) and information (e.g., radiocarbon dates) obtained by the other projects, but will in turn also provide additional insights to the investigation of the museums’ collections.